WISE 2019 Lecture and Laboratory Notes, Presentations and Videos
This web page provides links to materials for the WISE Summer Institute for 2019. This information is intended for the students participating in the 2019 WISE Summer Institutes. It is not intended for general public use and access is password protected. Access to these material is with the username/password students used to register on DSAP-WISE The material on these pages is provided for educational purposes only. Unauthorized use of this material is prohibited. If you have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University.
| Ch. | 2019 Lecture Topics Student Links |
Notes (.pdf) |
Figures (.pdf) |
|---|---|---|---|
| 0 | Introduction and overview of project (video) | Ch0.pdf | |
| 0 | Polymers and DNA Structure | Ch0.pdf | |
| 1 | Vectors: Plasmids, Libraries, and Plasmid DNA preps | Ch. 1 | Ch1.pdf |
| 2 | PCR, Mapping the size of an insert | Ch. 2 | Ch2.pdf |
| 3 | Restriction enzymes,Mapping the size of an insert | Ch. 3 | Ch3.pdf |
| 4 | DNA Sequencing, | Ch. 4 | Ch4.pdf |
| 5 | Logging onto DSAP, Sequence quality, cropping the ends | Ch. 5 | Ch5.pdf |
| 6 | Editing the waveform | Ch. 6 | Ch6.pdf |
| 7 | BLASTN (DNA vs DNA search) | Ch. 7 | Ch7.pdf |
| 8 | BLASTX (Translated DNA vs Protein search) | Ch. 8 | Ch8.pdf |
| 9 | Determining ORFs, BLASTP (Protein vs Protein search) | Ch. 9 | Ch9.pdf |
| 10 | Literature Search | Ch. 10 | Ch10.pdf |
| 11 | Compare Sizes of cDNA Insert | Ch. 11 | Ch11.pdf |
| 12 | Structural analysis on the Protein Data Bank (PDB) | Ch. 12 | Ch12.pdf |
| 13 | Next Gen Sequencing and Searching the TSA Database | Ch. 13 | Ch13.pdf |
| 14 | Investigating Protein Interactions | Ch. 14 | Ch14.pdf |
| 15 | Investigating Protein Expression | Ch. 15 | Ch15.pdf |
| 15 | Molecular Biology Experiments: What to do next | Ch. 16 | Ch16.pdf |
| 16 | Preparing a Poster | Ch17.pdf |
| Lab | WISE 2019 Laboratory Protocols | .pdf File | |
|---|---|---|---|
| 0 | Introduction to the WISE laboratory - Overview of the laboratory experiments (video) | Lab 0 | |
| 1 | Pipeting and weighing (video) Background notes for Lab | Lab 1 | |
| 2 | Determining DNA Concentrations | Lab 2 | |
| A)- DNA Spotting Assay Spot Template.ppt Google Docs Spot Assay Sheet | |||
| Entering information into Google Docs Clone Report Sheet (video) | |||
| B) Spectrophotometer | |||
| 3 | Plating a cDNA Library | Lab 3 | |
| 4 | Sterile technique and setting up ON cultures (video) | Lab 4 | |
| 5 | Setting Up a PCR Reaction with Bacterial Cultures | Lab 5 | |
| 6 | Running an Agarose Gel on the PCR Samples | Lab 6 | |
|
Pouring an agarose gel video. |
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| 7 |
Plasmid DNA Minipreps (video) |
Lab 7 | |
| 8 | Setting up the Restriction Digests of Plasmid DNA | Lab 8 | |
| 9 | Running an agarose gel for the restriction digests video | Lab 9 | |
| See videos in Lab 6 for how to set up, run, mock up, and upload an agarose gel FAQ: Is there enough DNA in my miniprep to sequence? (video) Restriction Digest Gel template (RDG-Template.ppt). |
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| Material Safety Data Sheets | |||
| Guideline for Handling Bacterial Cultures and Waste | |||
Parental Release Form (doc) (pdf)
Download FinchTV (external site) Download FinchTV (from WSSP site)