The following are recipes and MSDS sheets for components of the reagents used in the WSSP research project.
Acetic Acid
Agar
Agarose
EDTA
Ethidium Bromide (EtBr)
Ethanol
H2O
Miniprep Soln I
Miniprep Soln II
Miniprep Soln III
Miniprep Wash Buffer
Miniprep Elution Buffer
NaAc (Sodium acetate)
NaCl (Sodium chloride)
NaOH (Sodium hydroxide)
SDS
TAE (Gel running buffer)
TE
Tris (for TE)
Tryptone (for LB media)
Yeast Extract (for LB media)
Recipes for WSSP reagents:
LB Add the following to 800ml H2O, 10g Bacto-tryptone. (1% final); 5g yeast extract. (0.5% final); 10g NaCl. (1% final)
Adjust pH to 7.5 with NaOH. Adjust volume to 1L with dH2O. Sterilize by autoclaving
Miniprep Soln I: 100 mM Tris-HCl (pH 7.5); 10 mM EDTA
Miniprep Soln II: 0.2 N NaOH, 1% SDS
Miniprep Soln III: 0.3 M Na acetate
Miniprep Wash Buffer: 80% Ethanol
MiniprepElution Buffer: 10 mM Tris-HCl (ph 7.5), 1 mM EDTA
TAE Gel Buffer (http://biotech.about.com/od/buffersandmedia/ht/TAE.htm)
Make a concentrated (50x) stock solution of TAE by weighing out 242 g Tris base (FW = 121.14) and dissolving in approximately 750 mL deionized water. Carefully add 57.1 ml glacial acid acid and 100 mL of 0.5 M EDTA (pH 8.0) and adjust the solution to a final volume of 1 L. This stock solution can be stored at room temperature. The pH of this buffer is not adjusted and should be about 8.5.