2024-2025 WSSP Teacher Links
This web page provides links to materials for the Waksman Student Scholars Program for 2024-2025 This information is intended for the faculty participating in the program during the 2024 summer institute and through the 2024-2025 academic year. access is only through by sharing through your. The material on these pages is provided for educational purposes only. It is not intended for general public use and unauthorized use of this material is prohibited. Unlike the files for students, which are password protected, these are accessible only if you have been shared. If you are not a member of the WSSP and wish to download the files or have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University.
For Powerpoint files without clicker questions, please see the Lecture and Lab links for Students
Problem Sets. The problem sets for the lectures, labs, and videos were developed by WSSP teachers and staff as part of previous WSSP Summer Institutes. There are separate answer keys (PS#A). Problem Sets 2 and 3 for each topic require a higher level understanding of the material.
Ch. (notes) |
2024-2025 Lecture Topics | Figures with Clicker Questions | Problem Set 1 |
Problem Set 2 |
Problem Set 3 |
---|---|---|---|---|---|
Teacher Meeting Powerpoint 9/17/24 | Mtg.ppt | Video | |||
Introduction and overview of project | Intro.ppt | ||||
0 | The Basics of Molecular Biology | Ch0Q.ppt | Video Worksheet | ||
1 | Vectors: Plasmids, Libraries, and Plasmid DNA preps | Ch1Q.ppt | Ch1-PS1A | Ch1-PS2A | Ch1-PS3A |
2 | PCR, Mapping the size of an insert | Ch2Q.ppt | Ch2-PS1A | ||
3 | Restriction enzyme, Mapping the size of an insert | Ch3Q.ppt | |||
4 | DNA Sequencing, Logging onto DSAP | Ch4Q.ppt | Ch4-PS1A | Ch4-PS2A | |
5 | Sequence quality, cropping the ends | Ch5Q.ppt | |||
6 | Editing the waveform | Ch6Q.ppt | |||
7 | BLASTN (DNA vs DNA search) | Ch7Q.ppt | |||
8 | BLASTX (Translated DNA vs Protein search) | Ch8Q.ppt | |||
9 | Determining ORFs, BLASTP (Protein vs Protein search) | Ch9Q.ppt | |||
10A | Literature Search (no clicker questions) | Ch10.ppt | |||
10B | Human Homolog Search | ||||
11 | Comparing the Predicted Size of the Inserts | Ch11Q.ppt | |||
12 | Search of the PDB and Structure Analysis (no clicker questions) | Ch12.ppt | |||
13 | Next Generation Sequencing and searching the TSA (no clicker questions) | Ch.13.ppt | |||
14 | Searching for Protein Interactions (no clicker questions) | Ch14.ppt | |||
15 | Expression and Localization Analysis | Ch15Q.ppt | |||
16 | Molecular Biology Experiments: What to do next | Ch16Q.ppt | |||
17 | Multiple Sequence alignment and structure analysis (no clicker questions) | Ch17.ppt | |||
18 | Analysis of REV sequences: determine overlap, contigs | Ch18Q.ppt | |||
19 | Spanning the gap between FOR and REV sequences | Ch19Q.ppt | |||
20 | Protein modeling and Jmol (no clicker questions) | Ch20.ppt | |||
21 | Preparing a poster (no clicker questions) | Ch21.ppt | |||
Teacher Review of DSAP | DSAP-Review | Video |
Teachers: Please sign the Clone Box Verification Form so that the clones in the box are in the proper location when you send in your box of clones to be sequenced.
Lab
|
Lab Activity
|
Problem Set 1
|
Problem Set 2
|
|
---|---|---|---|---|
Lab 0 | WSSP Project and Lab Set Up | LabSetUp.ppt | ||
Lab 1 | Pipeting, Metric System (video) | |||
Lab 2 | Sterile Technique, Setting up ON | L2-PS2A | ||
Setting up an ON video | VWS2.1A | |||
Google Docs video | VWS2.2A | |||
Lab 3AB | Process ONs -Step 1 of MP | L3-PS1A | ||
Lab 3C | Set up PCR | |||
Lab 4 | PCR Gel Analysis | L4-PS1A | L4-PS2A | |
Pouring a gel video | VWS4.1A | |||
Loading a gel video | VWS4.2A | |||
Mocking up a gel video | VWS4.3A | |||
Lab 5 | Plasmid DNA Minipreps (video) | L5-PS1A | VWS5A | |
Lab 6 | Setting up Digests | L6-PS1A | ||
Lab 7 | RD Gel Analysis (video) | L7-PS1A |
DSAP Teacher Review Video
This video describes the resources and steps teachers can use to monitor their students' activity on DSAP or review their students' clones.
Links to Grant information