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Lab Notes

2020-2021 WSSP Laboratory Notes and Videos

This web page provides links to materials for the Waksman Student Scholars Program for 2020. This information is intended for the students and faculty participating in the program during the 2020 summer institute and through the 2020-2021 academic year. It is not intended for general public use and access is password protected. The material on these pages is provided for educational purposes only. Unauthorized use of this material is prohibited. If you are not a member of the WSSP and wish to download the files or have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University.

Lab 2019 Laboratory Protocols Laboratory Protocols .pdf  Videos 
0 Introduction to the WSSP laboratory - Overview of the laboratory experiments Lab 0 Lab 0
1 Pipeting and weighing Lab 1 Lab 1
2 Sterile technique and setting up ON cultures  Lab 2 Lab 2A
Lab 2B
  Entering information into Google Docs Clone Report Sheet
3A/B Processing cells from an ON culture to use in PCR and Minipreps at a later date Lab 3A/B  
3A Setting Up a PCR Reaction with Bacterial Cultures
NOTE: Some schools will be using a 2X PCR Mix while other schools will be using PCR beads.  Please be sure that you are using the correct protocol!   
Lab 3Ai-Mix
Lab 3Aii-Bead
 
 
3B Running the first steps of the Plasmid DNA Miniprep
NOTE: Most schools will be using plasmid miniprep reagents from Qiagen while other schools will be using plasmid miniprep reagents from GE.  They use different volumes of the reagents  Please be sure that you are using the correct protocol!   
Lab 3Bi-Qiagen
Lab 3Bii-GE
 
4 Running an Agarose Gel on the PCR Samples Lab 4  
   

A. Pouring an agarose gel 
B. Loading an agarose gel  Examples of gel loading video.
C. Mocking up a gel image in Powerpoint
PCR Gel template (PCRG-Template.ppt)
D. Uploading a gel image to Google Docs Report sheet 
PCR Gel Analysis Video

 

Lab 4A
Lab 4B

Lab 4C

Lab 4D

 

 

5

Plasmid DNA Minipreps
NOTE: This year some schools  will be using Qiagen solutions while others may be using GE Solutions.  Please check with your teacher which set of solutions your are working with and which protocol to follow.

Lab 5i (Qiagen)
Lab 5ii (GE)
Lab 5
6 Setting up the Restriction Digests of Plasmid DNA Lab 6  
  See videos in Lab 4 for how to set up, run, mock up, and upload an agarose gel
7 Running an agarose gel for the restriction digests Lab 7 Lab 7
  FAQ: Is there enough DNA in my miniprep to sequence?
Restriction Digest Gel template (RDG-Template.ppt). 
  Download 4-Peaks (from WSSP)    
  Material Safety Data Sheets    
  Guideline for Handling Bacterial Cultures and Waste    
  Required equipment and supplies