2020-2021 WSSP Laboratory Notes and Videos
This web page provides links to materials for the Waksman Student Scholars Program for 2020. This information is intended for the students and faculty participating in the program during the 2020 summer institute and through the 2020-2021 academic year. It is not intended for general public use and access is password protected. The material on these pages is provided for educational purposes only. Unauthorized use of this material is prohibited. If you are not a member of the WSSP and wish to download the files or have comments or questions about the material please contact Sue Coletta, Waksman Institute, Rutgers University.
| Lab | 2019 Laboratory Protocols | Laboratory Protocols .pdf | Videos | |
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| 0 | Introduction to the WSSP laboratory - Overview of the laboratory experiments | Lab 0 | Lab 0 | |
| 1 | Pipeting and weighing | Lab 1 | Lab 1 | |
| 2 | Sterile technique and setting up ON cultures | Lab 2 | Lab 2A Lab 2B |
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| Entering information into Google Docs Clone Report Sheet | ||||
| 3A/B | Processing cells from an ON culture to use in PCR and Minipreps at a later date | Lab 3A/B | ||
| 3A | Setting Up a PCR Reaction with Bacterial Cultures NOTE: Some schools will be using a 2X PCR Mix while other schools will be using PCR beads. Please be sure that you are using the correct protocol! |
Lab 3Ai-Mix Lab 3Aii-Bead |
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| 3B | Running the first steps of the Plasmid DNA Miniprep NOTE: Most schools will be using plasmid miniprep reagents from Qiagen while other schools will be using plasmid miniprep reagents from GE. They use different volumes of the reagents Please be sure that you are using the correct protocol! |
Lab 3Bi-Qiagen Lab 3Bii-GE |
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| 4 | Running an Agarose Gel on the PCR Samples | Lab 4 | ||
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A. Pouring an agarose gel |
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| 5 |
Plasmid DNA Minipreps |
Lab 5i (Qiagen) Lab 5ii (GE) |
Lab 5 | |
| 6 | Setting up the Restriction Digests of Plasmid DNA | Lab 6 | ||
| See videos in Lab 4 for how to set up, run, mock up, and upload an agarose gel | ||||
| 7 | Running an agarose gel for the restriction digests | Lab 7 | Lab 7 | |
| FAQ: Is there enough DNA in my miniprep to sequence? Restriction Digest Gel template (RDG-Template.ppt). |
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| Download 4-Peaks (from WSSP) | ||||
| Material Safety Data Sheets | ||||
| Guideline for Handling Bacterial Cultures and Waste | ||||
| Required equipment and supplies | ||||